The Insertion of L. hesperus TuSp1 gene into B. mori

TuSp1 molecular structure (solution structure of TuSp1 domain) The amino acid sequence of TuSp1 gene contains a signal peptide adjacent to the non-repetitive sequence of NTD at the N-terminus. Nonrepeating sequences are also present at the C-terminus (CTD). Associated with CTD are two iterations of RP 2 type. There are also 20 identical type 1 iterations (RP 1). Panels A to H represent different TuSp1 gene domains. Photographs A to D represent NTD, RP1, RP2, and CTD domains, respectively.

Mouse and mouse results can be obtained by allowing researchers to insert genes or, in particular, to eliminate new techniques specifically targeting genes. Behavioral differences in transgenic or knockout mice compared to mice without insertion or deletion means the effect of that particular gene on behavioral characteristics. These techniques also allow researchers to identify specific aspects of the function of specific genes and the function of these genes is unmeasurable. For example, by knocking out genes encoding neuronal receptors to study new transcripts of serotonin, scientists increased their dependence on male mice. Similar studies will allow us to better understand the ability to understand the activity of specific genes.

Genetic engineering techniques involve the insertion of new genes into the host's DNA. The sequence and major functions of the genes in the insert are usually well defined, but the current method inserts the gene sequence into random positions of the host DNA. There is no external control of where the insert is integrated into the host DNA. This results in unpredictable expression of inserted and surrounding natural genes, as well as differences in the local environment of the inserted genes. Therefore, the inserted gene may cause an unexpected additional effect, which may be unexpected

Facts of genetically modified organisms suggest that genetic modification involves three basic processes: gene mutations, insertions or deletions. As the most invasive method, gene insertion is usually done by inserting genes from different species or by horizontal gene transfer. For various reasons, these processes occur spontaneously when foreign DNA penetrates the cell membrane of the target organism, artificially achieving the same effect can be achieved by attaching new genes to viruses and physically As shown in FIG. Go to the nucleus. Use target cells, electroporation (which directs DNA of one organism to the electrocapillary of another organism), use of gene gun, or modified natural form of gene transfer (eg introduction of genetic material into plants). Lentivirus, gene transfer into animal cells