The Enzyme Catalase Experiment

The purpose of the enzyme catalase experiment was to perform a series of experiments involving catalase to determine the properties of some enzymes. The enzyme has a reaction rate under different conditions. Change in enzyme concentration, pH change, temperature change, and effect of inhibitor at different concentrations were tested. Raising the enzyme concentration increases the reaction rate. The optimum pH and temperature of the enzyme is found and outside of this optimum the reaction rate will be slow.

Throughout the report you will get information on how the temperature affects the amount of oxygen produced by the enzyme-catalase experiment. In the experiment, a liver extract was used as the catalase, a chemical reaction occurred in the reaction chamber between catalase and hydrogen peroxide and at three different controlled temperatures. The following procedure explains how to create an experiment. As a result, it was found that the higher the temperature in the water bath, the more oxygen is generated and "discharged" from the reaction chamber. In summary, the reaction chamber placed in the highest temperature water bath produced an average of 0.5 milliliters of oxygen than the reaction chamber placed in the lowest temperature water bath tested. In this year's scientific research, students have discovered information about enzymes and their work.

Activity of enzyme catalase and hydrogen peroxide Background information: Enzyme (catalase) is a biocatalyst. It can change the reaction rate without changing itself. Enzymes are also globular proteins with specific shapes in three dimensions; within pockets with active sites, they have the correct shape to connect to the correct substrate. - Influence of temperature on enzyme catalase activity Introduction: Hydrogenase is added to hydrogen peroxide (H 2 O 2), which reacts vigorously and produces oxygen. This experiment examined the effect of temperature on the rate of action of the enzyme by measuring the amount of oxygen generated over a period of time. Experiment to record the result by changing the temperature