The Effects of Enzyme Concentration on the Activity of Amylase

Influence of enzyme concentration on amylase activity The effect of amylase concentration on its activity was investigated. The relative activity of the amylase was found by noting the time it took for the starch substrate to break, ie when it was no longer blue-tinged when tested with iodine solution. This time it is called an achromatic point. Apparatus: v-amylase solution 0.1% v starch solution 0% v distilled water i iodine in potassium iodide solution v white ceramic tile and polyethylene pipette v graduated pipette or syringe v test tube on test tube v beaker (water bath Used as v) stopwatch, thermometer vs. P.

Effect of pH on the activity of the enzyme amylase Objective: - We have found the effect of pH on the activity of the enzyme amylase. Enzymes are proteins found in all living cells. - Examining the influence of temperature on the enzyme structure Introduction: To examine the enzyme amylase and substrate starch for the biological evaluation of my GCSE. The reaction that I am trying to study is called protein testing of starch. Purpose: My observation is to study how enzyme catalysis is influenced by temperature change.

Alpha amylase is an enzyme. It binds specifically to water and starch. It hydrolyzes starch and glycogen to produce glucose and maltose. It acts on alpha binding of polysaccharides. Due to the specificity of the enzyme activity, the structure of the enzyme must be accurate. Any factor that causes enzymatic denaturation will limit its activity. The two most important factors that influence alpha amylase activity are temperature and pH. The following experiment was conducted to investigate the effect of different ambient temperature and pH on the activity of barley amylase.

The enzyme accelerates the reaction rate by lowering the activation energy. Each enzyme works better under optimal conditions, which supports the most active form of the enzyme molecule. Enzyme and substrate concentration, temperature and pH are important environmental factors by producing maximum reaction rate. In addition, different factors were used to examine the effect of catalase and human amylase. Gas pressure sensor, Vernier gas pressure port and Logger Pro are used to collect oxygen pressure. At 30 ° C. and pH 7 of catalase, substrate and enzyme concentrations increase and the reaction rate increases. However, the substrate concentration increased with human amylase at 40 ° C. and pH 7.

The figure shows the relationship between the reaction rate of human amylase and pH. Data were collected from simulation experiments using computer software Enzyme Investigations. When the temperature is 250 ° C., the substrate concentration is 0.01 mol / l and the enzyme concentration is 0 × 10 -6 mol / l, so the pH varies between 1 and 14. Relationship between reaction rate and substrate concentration of human amylase Data was collected from simulation experiments using computer software Enzyme Investigations. In this experiment, the temperature was kept constant at 40 ° C., the enzyme concentration at 0 × 10 -6 mol / l, and the pH at 7.