The Effect of Temperature on the Activity of Rennin in Milk

Influence of temperature on renin activity in milk Objective: To understand the influence of various temperatures on renin in cow milk. Introduction Enzymes are biocatalysts. It accelerates the reaction by reducing the activation energy required to initiate the reaction. The reaction will be faster but as long as there is no limitation, it will be the same. It consists of amino acid polymers. The enzyme has the highest pH and temperature. When enzymes are in optimum conditions, the reaction rate is fastest.

Renin is an enzyme that cures milk. You can test the effect of Renin on milk in a simple experiment. 3 ml of milk was placed in 3 tubes, 4 ml of chymosin was placed in 3 tubes, and 1 milk and 1 chymosin tube were placed in each water bath for 10 min. You can use the same water bath as amylase experiment. Renin was added to each milk tube and the tube was returned to the water bath for 10 minutes. You will notice that reaction at room temperature will produce solidified milk, but the contents of the tube in a cold water bath will be somewhat hard and the contents of the tube in hot water will not cure.

Part 3: Determine the effect of temperature on lactase activity • Milk should be at the desired temperature before adding enzyme. • If you do not use the ice cube and place it in a nearby refrigerator, you can store it on the shelf. • Water bath with adjustable heater or hot plate with 1000 ml glass beaker The To Lactase laboratory is an activity to promote understanding of enzymes and activities and factors influencing them. It also combines laboratory science and medical biotechnology research. The lab consists of three parts. Part 1: Checking the type of milk The student confirms that Lactex can break down the milk sugar. Part 2: Determining the effect of pH on lactase activity Students study the effect of pH on enzymatic activity. Part 3: Determining the effect of temperature on lactase activity Students study the effects of temperature on enzymatic activity