The Effect of Activator Concentration on the Rate of Reaction of Fungal Amylase

Effect of active agent concentration on fungal amylase reaction rate Objective To investigate the influence of activator concentration (calcium) on the rate of starch based enzyme (fungal amylase) reaction. INTRODUCTION Enzymes are composed of globular proteins, each of which has a special complicated form complementing the matrix to be destroyed. The shape of the enzyme and its active site is due to the specific folding of the polypeptide chain therein.

The purpose of this experiment was to study the enzymatic kinetics of the starch hydrolysis reaction with the enzyme, alpha-amylase and to determine the kinetic effect of the alpha-amylase concentration change on the reaction rate over time there were. As shown in the table, the experiment was carried out in three main phases. For alpha-amylase concentration, experimental data on the time course of the reaction between the alpha-amylase and the starch-iodine complex were obtained at 0.02% w / v, 0.01% w / v and 0.005 It was. Absorbance of% w / v starch-iodine complex was read spectrophotometrically every 5 minutes every 5 minutes. A series of calculations were performed to infer the amount of product produced and a progress curve was generated for each alpha-amylase concentration during the reaction, in the figure, the initial reaction rate = 0 to time = 2 is the tangential line from time Can be plotted. Then calculate the gradient

Table 6: Effect of different enzyme concentrations on the reaction rate between alpha-amylase and starch. This table emphasizes that as the reaction of a specific alpha-amylase concentration progresses, the volume of the product increases with time and the prominent P is proportional to the level of product formation. Also, as the concentration increases with α-amylase, P also increases at constant time intervals, a progress curve can be drawn from the data in Table 6, and the initial reaction rate (V 0) of each α-amylase concentration in the figure is calculated can do. The initial reaction rate is inferred by plotting the tangent of the progress curve for a particular alpha-amylase concentration from the origin 0, 0 and extrapolating to T = 2; then calculating the gradient to obtain the initial reaction rate.