The Development of Primers for Unknown Thermophilic Bacteria at Vulcan Hot Springs

The aim of the Thermus Aquaticus project was to identify thermophilic bacteria in the local hot spring Balkan hot spring by gene sequencing and analysis. Other Thermus bacterial polymerase genes have been shown to be useful for genetic responses. Vulcan bacteria grow at higher temperatures than other thermophilic bacteria, giving the possibility of polymerase genes more efficient than currently available. My own research focused on designing efficient primers for bacterial polymerase genes.

Thermophilic bacteria are common in soils and volcanic environments, ie hot springs. The thermophile is thought to be one of the primitive organisms that survived the earth over 3 billion years ago, and in a much warmer environment this can be the life form found on other planets It is possible to assume. The ability to grow at a growth temperature of up to 60 ° C is associated with an extremely thermally stable macromolecule. Due to the proliferation at high temperature and unique macromolecular properties, thermophilic organisms can have high metabolic rate, physically and chemically stable enzymes, as well as lower growth rates and higher end product yields . The thermophilic reaction appears to be more stable, faster and cheaper and facilitates reactant activity and product recovery. Oxygen is difficult to dissolve at high temperatures, so organisms are not available, so most thermophilic organisms are anaerobic bacteria.

The thermophilic bacterium is a bacterium growing at a very high temperature, typically between 50 ° C. and 80 ° C. These bacteria are classified as facultative thermophilic bacteria and absolute thermophilic bacteria. Thermophilic bacteria called hyperthermophilic bacteria experience optimal growth only when it exceeds 80 degrees Celsius. One of the most remarkable findings is the discovery that the archaebacterium Thermas Aquaticus is strong against high temperatures. This is due to the survival mechanism found in Thermus Aquaticus, which makes it possible to replicate DNA at very high temperatures. Vibrio vlunificus is one of the curved bacteria that can survive in the marine environment. These bacteria grow very fast in warm seawater. Deinococcus radiodurans is another type of microorganism that can survive easily in the most harsh environments and emit high levels of radiation.