Temperture's Effect on Beet Membranes

Influence of temperature on sugar beet film Objective: To investigate the influence of temperature on membrane structure Assumption: The higher the temperature, the higher the amount of pigment emitted from beetroot cells. As the temperature rises, the kinetic energy of the molecule in the cell increases and differentially permeates the cell membrane, thereby increasing the rate at which the dye diffuses from the cell. As temperature rises further, the shape of protein molecules in the cell membrane changes.

In this experiment we investigate the effects of various temperatures on membrane permeability of beet (Beta vulgaris), commonly called red beet. You can use seven different samples and treat each sample to a different temperature and compare how the temperature affects the release of betaine released under normal conditions. The amount of betaine pigment released was measured spectrophotometrically at a wavelength of 475 nm. The increase in temperature was found to be related to the amount of β-cyanine dye passing through the membrane. For example, at 22 ° C, 60 ° C, and 100 ° C the absorbance values ​​are 0.0558, 285, and 401 respectively. This tendency strengthens the belief that the increase in temperature is proportional to the amount of beta anthocyanin that can pass through the membrane due to the increase in membrane fluidity.

When 1-propanol is the most polar nonalcohol of the alcohol being tested, 1-propanol will cause the greatest damage to the biofilm (vacuoles of beet cells). In this experiment, the effects of three different alcohols, methanol, ethanol and 1-propanol were tested in Biker Tonoplast. Absorbance measurements are collected using a conductivity probe. When the tonoplast (cell membrane) of the beet cell is damaged, the red pigment β-anthocyanidin leaks from the cell. The more red pigment that leaks into the surrounding environment, the stronger the pigment, the greater the amount absorbed by sugar beets and the damage to the cell. Results Different concentrations of alcohol solution were placed in a spectrophotometer in a plastic cuvette and the absorbance of methanol, ethanol and 1-propanol was collected.