Stomata Count Experiment
2023-06-27 15:48:39The purpose of the pore counting experiment was to peel off the epidermal layer from under the leaves of the plants and calculate the average distance between the pores. Background information Pores protecting cells from opening and closing absorb carbon dioxide required by plants to produce glucose by photosynthesis. Make them rough.
Pores are the main means of plant gas exchange. Pores are small holes controlled by protective cells to control the opening and closing of the pores. The vent allows carbon dioxide to enter the plants, allowing water and excess oxygen to escape. About 90% of the water is lost from the leaves during transpiration. Place the scale on the eyepiece of the microscope. This is done by loosening the top lens and placing the scale in the lens body. Then tighten the top lens. Place the stage micrometer on the stage and fix it with a clip. Observe through the microscope and focus 40 times so that you can clearly see the two scales. Carefully move the platform micrometer so that the starting units of the two scales match. Count along two scales until a match occurs again between the two scales. Please pay attention to the division number of each scale. I repeat the magnification of 100 times and 400 times
The photograph shows the percutaneous, sunken and shaded leaves of the sun and shadow, and the number of each pore. Transcutaneous sections of sunlight leaves indicated epidermal cells did not notice pores or veins. Shaded leaves have epidermis and veins. The cross section of the sun shows epidermis, small cuticle and some leafy meats. The shaded cross section shows epidermis, subcutaneous, epidermis and thick mesophyll. Each leaf has no pores in leaves or parietal lobes. The number of pores in sunrise was 109, 107, 98, 111, 106. The average number of vents was 106.2. The number of pores in the shaded leaves were 113, 97, 121, 108, and 114, respectively, the average was 111, the area of the leaves was divided by the area of 400 times, and then the number was multiplied by the number of pores The proportion of the skin was determined by things. The ratio of the epidermis to the pore of the sun leaves is 0.283. The proportion of leaves with shade is 03