Stability Indicating and Kinetic Determinations of Fluvastatin Sodium by RP-HPLC-DAD Method

5 Kinetic analysis 5.1 Kinetic studies of FVS in acidic degradation The acid decomposition kinetics of FVS were evaluated at 70 ° C. in 0.1 M HCl for various periods. A solution containing 1 mg / mL FVS was prepared in water. A suitable aliquot was transferred to a volumetric flask and diluted to a final concentration of 100 μg / ml FVS with 0.1 M HCl. The solution was heated to 70 ° C. and evaluated for time intervals of 30 minutes, 60 minutes and 120 minutes. Three samples were analyzed at each time interval.

In this study, a simple and stable indication, accurate and effective stability was developed and an HPLC method for the simultaneous quantification of ceftriaxone and sulbactam in its pharmaceutical formulation was shown. This method was validated according to FDA guidelines. CFX and SBM eluted at 3 and 6 minutes, respectively. The correlation coefficients (r 2) of CFX and SBM were found to be 0.996 and 0.9976, respectively. The lower limit of quantification (LLOQ) of ceftriaxone was 0.87 μg / mL, and that of sulbactam was 0.96 μg / mL. % CV <2 was found with accuracy during the day and day

Abstract A simple and accurate HPLC method for the determination of piroxicam in gels was developed. A reversed phase HPLC system consisting of a C18 column of 150 mm x 6 mm size was used. 55 volumes of methanol and 45 volumes of phosphate buffer (0.05 M, pH 7) were used as mobile phase. The flow rate was 1 ml / min and the effluent was monitored at 254 nm. The retention time was found to be about 0 minutes. A stock solution of piroxicam was prepared and a standard solution of 5-20 μg / ml was prepared using phosphate buffer × methanol (60 × 40, v / v). This was injected into HPLC to obtain a chromatogram. Test solutions were prepared from commercial product Feldene gels and injected into HPLC. The concentration of Velden gel was determined based on the regression equation obtained from the standard concentration.