Single-stranded binding protein

Single chain binding protein (SSB) is a class of proteins that have been identified in bacteria to human viruses and organisms.

It binds to single-stranded DNA to prevent denaturation of single-stranded DNA into double-stranded DNA, and prevents degradation of single-stranded DNA.

The overall situation of human cytomegalovirus (HHV - 5) DNA synthesis seems to be a typical feature of herpesviruses, but several new features emerge.

In ICP 8, herpes simplex virus (HSV - 1) single stranded DNA binding protein (ssDNA binding protein (SSB)) consists of 8 alpha helices. The front of the cervical area consists of 5 β sheets and 2 α helices, the back side is a 3-stranded β-sheet, and the shoulder of the N-terminal domain is composed of α helix and β helix. Tablespace [1] Herpes Simplex Virus (HSV-1) SSB, ICP 8 is a nuclear protein, together with other replication proteins, is required for viral DNA replication during lytic infection.

The six herpesvirus group co-genes encode proteins that can constitute a replication fork mechanism comprising a duplex subunit DNA polymerase, a helicase-primer complex, and a single stranded DNA binding protein. [2] Human herpesvirus type 1 (HHV-1) single-stranded DNA binding protein ICP 8 is a 128 kDa zinc metalloprotein. The photoaffinity label indicates that the region comprising amino acid residues 368-902 contains a single stranded DNA binding site for ICP8. [3] The HHHV-1 UL5, UL8, and UL52 genes encode the necessary heterotrimeric DNA helicase primers involved in concomitant DNA unwinding and primer synthesis on viral DNA replication forks. ICP 8 promotes unwinding of DNA and bypasses cisplatin-injured DNA by injecting a helicase primer into DNA. [Four]

Bacterial SSB protein domains are important for maintaining DNA metabolism, more specifically DNA replication, repair and recombination. [5] It has three β chain structures that form a 6-chain β sheet to form a duplex. [6]

Replicon A is a functional equivalent of SSB in the nucleus of eukaryotic cells, but there is no sequence homology

Eukaryotic mitochondria contain their own single stranded DNA binding proteins. Human mitochondrial SSB (mtSSB) binds to single-stranded mitochondrial DNA as a tetramer and has sequence similarity with bacterial SSB [7]. Human mtSSB is encoded by the SSBP1 gene. In yeast, it is encoded by the RIM1 gene. [8]

National Medical Library Graduate School of Medicine (MeSH) Single strand + DNA + binding + protein

MTB duplicates by bipartite fission. During DNA replication, DNA helicase binds to the DNA double helix and begins to unravel the parent strand by cleaving hydrogen bonds between base pairs using ATP. Single-stranded binding proteins stabilize unintercriminating DNA strands and help prevent them from re-pairing. The point where two DNA strands separate is called a replication fork. DNA polymerase then migrates along each strand of DNA behind each replication fork to synthesize new DNA nucleotides. As the copy branch expands, the forward supercoil starts to accumulate before the copy fork. In order to continue DNA replication, it is necessary to eliminate the positive supercoil. Super coil makes DNA more compact structure. DNA gyrase inserts a negative supercoil into Mtb DNA. DNA gyrase binds to circular supercoiled DNA molecules, which alleviates positive supercoils. Therefore, a DNA molecule with a positive supercoil will now have a negative supercoil.

A unique set of DNA binding proteins are DNA binding proteins that specifically bind to single stranded DNA. In humans replicating protein A is the most comprehensible member of this family and is used to separate duplex processes including DNA replication, recombination and DNA repair. These binding proteins appear to stabilize single-stranded DNA and protect it from stem-loop formation or breakdown by nuclease. Instead, other proteins have evolved to bind to specific DNA sequences. These most detailed studies are various transcription factors that are transcription regulating proteins. Each transcription factor binds to a specific set of DNA sequences and activates or inhibits transcription of genes having these sequences close to their promoters. Transcription factors do this in two ways

Eukaryotic mitochondria contain their own single stranded DNA binding proteins. Human mitochondrial SSB (mtSSB) binds to single-stranded mitochondrial DNA as a tetramer and has sequence similarity with bacterial SSB. Human mtSSB is encoded by the SSBP1 gene. In yeast, it is encoded by the RIM1 gene.