Science Project Using Paper Chromatography

The purpose of this project is to use paper chromatography to check which dyes are used in the coating of your favorite colored candy. Why are different compounds traveling different distances on paper Why is the Rf value useful? What is the chromatogram used for? Candy & color paint - at least 30 sheets of skittles and M & M - coffee filter or chromatography paper 3 cm x 9 cm wide mouth bottle pencil ruler tape water toothpick food coloring (red, green, blue) The above terms, concepts and The problem is well known.

There are four kinds of chromatographs. These are liquid chromatography, gas chromatography, thin layer chromatography and paper chromatography. The world uses liquid chromatography to test water samples for lake and river pollution. It is used for analysis of metal ions and organic compounds in solution. Liquid chromatography uses a liquid that can incorporate hydrophilic insoluble molecules. Gas chromatography is used at the airport to detect bombs and is used for evidence in various ways. It is used to analyze human body fibers and analyze blood found at crime scene. In gas chromatography, hydrazine is used to move the gaseous mixture through the column of absorbing material. Thin layer chromatography uses absorbing materials on flat glass or plastic plates. This is an easy and quick way to check the purity of organic compounds. It is used to detect pesticides and residual pesticides in food. We use a piece of paper as stationary phase

Chromatography is a science study of molecular separation based on its structure and compositional difference. Typically, chromatography involves moving a blend of materials to be separated, "test formulation", "on a fixed support". The molecules in the test formulation have different interactions with the immobilized support so that similar molecules separate. Test molecules that show a more intimate interaction with the support are more likely to migrate through the support than those with weak interactions. In this way, when different types of molecules travel over the support material, they can be separated from one another.

Chromatography is a common method for characterizing, isolating and purifying molecules. Over the years, modern chromatography has automated and robotized, including many different types of chromatography including gas chromatography (GC), high pressure liquid chromatography (HPLC), and high performance liquid chromatography (FLPC). These systems are coupled to various types of detectors. Some examples refer to LC (liquid chromatography) by mass spectrometry (MS), UV / Vis, ultraviolet (UV) or visible (Vis) light absorption, fluorescence based detectors using many biomolecules. , Measuring fluorescent signals from fluorescent molecules that react or adhere to or bind various biomolecules, etc.