Role in Inducing and Maintaining the Pluripotency of Mice Embryonic Stem Cells

Several members of the zinc finger protein family are important building blocks in transcriptional networks that maintain pluripotency of embryonic stem cells. Mouse zinc finger protein 322a (Zfp 322a) is expressed in embryonic stem cells and ICM. So Ma et al. In 2014, Zfp 322a was thought to function as a transcription factor in mice. Ma et al. (2014) Zfp 322a is an irreplaceable transcription factor that enhances reprogramming ability of OKSM (Oct4, Klf4, Sox2 and c-Myc) and can replace Sox2 of OKSM's induced pluripotent stem cell (iPSC) discovered.

Stem cells including embryonic stem cells (ESCs), adult stem cells, and induced pluripotent stem cells (iPSCs) are useful in the field of regenerative medicine due to their pluripotency and self-renewal. In addition, stem cells have beneficial effects such as paracrine action, anti-inflammatory action and immunomodulatory ability. However, stem cell transplantation for the treatment of cardiac disorders is of limited potential due to their potential to differentiate into host cell types such as cardiomyocytes or vascular cells that interfere with cardiac function by causing arrhythmia, and their low potential under harsh conditions Treatment and survival are hampered. To overcome these problems, the damaged tissue needs to establish a new strategy to start stem cells.

There are three types of stem cells: embryonic stem cells, adult stem cells, artificial pluripotent stem cells (iPS). The most famous type of stem cells are embryonic stem cells. This is due to the extensive coverage of such stem cells by the media and the many judiciary laws developed based on their use. Because they are the most flexible stem cell types, they are also popular among researchers. Unlike their more mature counterparts, they have the ability to regenerate all types of cells. Embryonic stem cells are found in two areas: umbilical cord and embryo. Embryonic stem cells are found in blood taken from umbilical cord immediately after birth. It contains hematopoietic stem cells that can be used to produce erythrocytes; among other things. There are also mesenchymal stem cells that can be used to grow muscle and bones. Hematopoietic cells are used for the treatment of blood related diseases such as autoimmune diseases and leukemia

Recently, two different groups of scientists induced the molecular cell to return to the pluripotent state by molecular manipulation and produced "induced pluripotent stem cells" (iPS). Surface Markers and Protein Forms 4-8 Both groups use retroviruses to carry transcription factor genes to adult cells and these genes are transcribed and converted into proteins that regulate the expression of other genes. Since both introduce embryonic transcription factors called Sox 2 and Oct 4, it is attempted to introduce genes into cell purification methods together with safer transcription factors. The result looks promising