Relative and Absolute Quantitative Real-Time PCR

Relative and Absolute Quantitative Real-Time PCR (qRT-PCR) Introduction Quantitative real-time (qRT-PCR) polymerase chain reaction (PCR) in PCR is an innovative technique for molecular biological detection and DNA amplification Yes, it produces numbers. Copies of thousands to millions of specific DNA sequences. Quantitative real-time reverse transcription polymerase chain reaction PCR (RT-QPCR) is considered to be the most standard technique in molecular biology and is considered as a benchmark marker for detection of DNA and mRNA, and this technique is widely used It has been. Various fields of bioanalytical science (Burns et al., 2005).

However, the PCR method is readily applicable to the analysis of VNTR, in particular the STR locus. In recent years, the focus of quantitative research on human DNA has focused on new "real time" quantitative PCR (qPCR) technology. Quantitative PCR allows for automated, accurate, high-throughput measurements. Laboratory research demonstrated the reliable interpretation of STR typing and the importance of human DNA quantification to achieve laboratory consistent results. Currently used DNA analysis systems are based on polymerase chain reaction (PCR) and use simple sequences or short tandem repeats (STR). This method uses highly polymorphic regions with short repetitive DNA sequences (most commonly repeating 4 bases, but using other lengths including 3 and 5 bases). Since irrelevant people almost certainly have different numbers of repeat units, STR can be used to distinguish irrelevant people.

Real time polymerase chain reaction or qualitative polymerase chain reaction is a laboratory technique that not only amplifies but also quantifies the target DNA molecule. This helps the user to detect and quantify many specific genes (absolute and relative) present in a particular DNA sample. The general principle of polymerase chain reaction (PCR) is also used for RT PCR. Unlike PCR, RT PCR is a new technology product detected at the end of the reaction. RT PCR is used to amplify reversibly transcribed cDNA products from mRNA and it is becoming an effective and powerful tool in the field of molecular biology for quantification and research of gene expression . RT PCR methods are very easy to apply and provide reliable, accurate and rapid quantification of gene expression. Accurate nucleic acid quantification requires mathematical applications for data analysis (Michael W. P., 2001)