Pseudomonas Aeruginosa

Pseudomonas aeruginosa is a bar-like gram negative aerobic bacterium belonging to the family Pseudomonas aeruginosa. Pseudomonas aeruginosa is a common living bacterium commonly found in soil, water, sometimes plant surfaces and animal normal flora (Todar, 2008). As an opportunistic pathogen in humans it infects almost any damaged tissue and causes various infections caused by urinary tract infections, bacteremia and various systemic infections, especially infectious diseases with immune system disorders (Todar , 2008). .

Pseudomonas aeruginosa is a free-living bacterium commonly found in soil, water, bacterial flora in the skin and all artificial environments. Living organisms often occur on the surface of plants and animals. Pseudomonas aeruginosa is an accidental pathogen of plants. It is also an increasingly emerging clinically relevant pathogen. Organisms do not grow in the normal atmosphere and also grow in the hypoxic region to form colonies in both the natural environment and the artificial environment. Pseudomonas aeruginosa is a human opportunistic pathogen, meaning that it uses several breakthroughs in host defense to induce infection. Bacteria do not infect uninfected tissues, but if they contain tissue protection they infect bacteria. Urinary tract infections, respiratory infections, soft tissue infectious dermatitis, gastrointestinal tract infections, etc.

Pseudomonas. Pseudomonas aeruginosa is an opportunistic pathogen that may cause a fatal infection in a damaged host. This toxicity is related to the secretion of several extracellular proteins. Pseudomonas aeruginosa secretes two proteases, alkaline protease and elastase. The alkaline protease gene (apr) from P. aeruginosa IFO 3455 and PAO 1 was cloned into E. coli. A DNA fragment encoding alkaline protease derived from the PAO1 strain (8.8 kbp) was expressed in E. coli under the control of the tac promoter. The active enzyme was found to be synthesized without cell lysis and secreted into the medium.

Eps of Pseudomonas aeruginosa consists of polysaccharides, proteins and nucleic acids. It is an important part of biofilm. The exopolysaccharide genes lec A, Pil B, Psl A, alginate are involved in the formation of Pseudomonas biofilm. The Ps1 extracellular polysaccharide locus encodes bacterial cells to the surface to maintain the biofilm structure. The matrix immobilized by Psl is not directly visualized at the developmental stage of Pseudomonas aeruginosa biofilm