Procedure to Investigate the Effect of Temperature on Beetroot

Objective: To study the influence of temperature on cell membrane structure. Hypothesis: As the temperature rises, we anticipate that more concentrations of betaine will be released. This is because the temperature softens cell vacuoles and cell membranes, allowing more betaines to pass through. Introduction: The beetroot contains a red pigment called betaine. Normally pigments do not pass through the membrane, but when the beetroot is cooked they leak out. For proper functioning, the cells must be able to control the transport through the partially permeable membrane.

In order to investigate the influence of temperature, the following experiments were conducted on the beet root tissue, observing that the red pigment was removed from the cells at different temperatures, to ascertain whether the above theory is correct, and to check around the beat root at high temperature Red pigment penetrates into. Areas 6.15 test tubes, these are used to put a beat route in a water bath, 15 is necessary, because the samples of three beat routes are averaged over each temperature for more accuracy for five different temperatures These are used instead of boiling tubes or other equipment as they can be loaded in a water bath so that they can be loaded into the colorimeter to measure the transport of their water color.

Since we are experimenting with the influence of temperature on the membrane, the beat root samples were placed in a water bath at different temperatures and the change in color in water was measured. Temperature is only one of the possible variables. Others are affected by harmful substances such as alcohol and various solvents. The dependent variable (DV) in this experiment is the color change in water due to anthocyanin leakage. Beat routes must be prepared first before starting the experiment. To do this you need a white tile and core. The surface area of ​​each beat root part is very similar as all parts need to use koalas of the same diameter. To collect bead root cylinders, simply push the koala into the vegetables and remove it. Since the cylinder remains in the coreer, it is necessary to extrude using a smaller diameter coreer. If you collect some good, uniform cylinders, you must cut them into seven cylinders of equal length.