Influence of various substrates on population growth and population growth plan of yeast: Introduction: Yeast is a unicellular fungus vegetatively propagating by budding or division, such as Saccharomyces, and is important in food fermentation (walker, 1998). ) Yeast is widely distributed naturally. Like bacteria and molds, they can have properties that are beneficial and ineffective in food production. The most famous examples of yeast fermentation are the production of alcoholic beverages and the fermentation of bread.
Experiments were conducted to study the population growth of yeast Saccharomyces cerevisiae under various environmental conditions such as temperature, pH level and glucose concentration. Then the problem was studied: What is the influence of different temperatures on the growth of Saccharomyces cerevisiae? The concentration of glucose in the culture used to test the effects of temperature and pH is chosen so that with respect to glucose the yeast population can grow without limitation. One option for testing the influence of glucose on the growth of yeast is the absence of glucose in the culture. Another option is to halve the optimal glucose concentration, the last one being the best value.
S. cerevisiae at different glucose concentrations. Analysis of the growth of S. cerevisiae and the fermentation of 24 hours showed that the yeast population did not increase at all in the absence of glucose from the culture. The only increase observed from that initial population is 091.1 times, which means that this increase can be 0.1 due to the energy capacity in the yeast cells. Sufficient growth was observed at 1% glucose concentration. The initial value of the yeast population almost doubled (about 8-fold increase). At higher glucose concentrations, the yeast cell population responded more, so higher population growth was observed. The initial population has increased nine times. In other words, it has almost doubled. The population increased by 954.00 times, almost the same as the 2% concentration. Therefore, the marginal growth glucose concentration was 2%. Overall, this assumption has been confirmed
Evaluating the growth of the yeast population at different pH levels shows that the increase in population above and below pH 6 is nearly identical. In fact, the highest population growth observed at pH 6 means that this is the optimum pH level. The lowest growth observed at pH 3 and pH at these particular pH levels, growth at pH 8 (about 7 times increase in population) is slightly less than growth at pH 3 (about 3-fold increase in population) it was high. If the growth is higher, the pH is 8 because it is close to the optimum pH. At pH 4, the pH increased to about the same, pH values at pH 4 and pH 8 differed by optimum value and 2 pH value, but the yeast population at pH 4 increased about 982 times, the population at pH 8 increased. Make it 7 times. This indicates that S. cerevisiae performs better under acidic conditions. Overall, this assumption has been confirmed