Microscopy

Microscope summary The whole exercise is about care and use of the microscope. We are doing a variety of activities to enable us to become familiar with the components and applications of the microscope. Using a microscope, we observed cotton, silk, hair, and even various objects like the letter "e". In order to observe it, we use a low magnification objective lens and a high magnification objective lens. These activities were done to better understand how the compound optical microscope works and to understand the various procedures for setting slides.

Scanning electron microscopy (SEM), transmission electron microscopy (TEM) and atomic force microscopy (AFM) are the three most commonly used microscopic techniques for visualizing these complexes. They generally indicate that most composite materials are discrete spherical nanoparticles with a smooth surface. AFM does not show aggregation in the liquid environment, suggesting that these PECs may be stabilized by charge repulsion. Interestingly, other forms such as vesicles, nanoparticles with dense nuclei, and nanoparticles with the appearance of "fluffy" have also been reported. Unlike most composites formed between polyelectrolytes and proteins, these PECs with different morphologies are formed by amphiphilic polyelectrolytes. The presence of hydrophobic moieties on these polyelectrolytes is believed to have a major impact on the morphology of the complexes thus produced.

The low-temperature electron microscope is a method of imaging a frozen hydrated sample with an electron microscope at low temperature. The specimen maintains its natural state without the need for dyes and fixatives and can examine the cell structure, viruses and protein complexes with molecular resolution.

Electron microscope - use focused electron beam instead of light. Electron microscopes allow higher sample magnifications than optical microscopes and can be used to obtain detailed information about intracellular structures. Electron microscopy requires a lot of processing and can therefore only be done on fixed samples. The transmission electron microscope provides a cross section of the sample and the scanning electron microscope provides a three dimensional image of the sample surface.

Basically, the low temperature electron microscope (Cryo-EM) is a transmission electron microscope that makes it possible to observe the target sample at low temperature. After years of refinement, low temperature electron microscopy has become a useful tool for observing and studying the structure of various biomolecules. Because photons are energy packs (the basic particles of light), people see in their eyes because these particles reflect objects we are perceiving and objects that enter our eyes. However, some objects (in this case, samples) are too small compared to the photon wavelength so that they can not interact and therefore can not be observed.