Discussion on microbial contamination in the meat depends on how the animal is slaughtered and removed, and how meat is usually handled and stored in terms of time and temperature. They also depend on sterilization conditions of slaughterhouses (Brown and Baird-Parker, 1982). Chemicals, salts, low temperature, heating and radiation practices have been used to eradicate and slow down or reduce pathogens in food and food. Organic agents for antimicrobial organic acids such as lactic acid and acetic acid have been used as food preservatives and have been shown to be generally safe (GRAS) (Branen et al., 1990) .
In addition, handling and storage of beef effectively promotes meat contamination (Beinempaka et al., 1990). The storage location must be clean, preferably cold (400 F). Any of the above temperatures will cause microbial growth, which will affect meat and cause deterioration, and beef must be properly handled. Because all necessary safety and health considerations are on slaughterhouses or farms, animals slaughtered at slaughterhouses and mature farms are usually less bacteria than meat from animals slaughtered in the backyard. Therefore, unlike animals slaughtered in the backyard, the degree of infection is kept to a minimum. Animals are killed without becoming hygienic and can be put on the floor (Zeigler, 1966).
Cx microbial contamination fuzzy count c microbial contamination does not hide count incomplete lawn (toxic) mc small colony (toxic) nl no turf (toxic) pc sediment coverage number pol sediment fuzzy evaluation background turf ppt deposition Object vc visual count vr view recalculation Note: Standard deviation of SD sample, NA does not apply to lawn with T low recovery colony number (multiple reaction, 0.6) or incomplete / no background (il or nl There is no meaningful counting result on the plate that contains) Because it is unknown that the dose based on past control values is relevant and not out of the normal range due to normal fluctuation rather than toxicity, it is less counted It is considered to be. II), no turf (nl), sediment (ppt), no fuzzy count of contamination (c), and fuzzy evaluation of background turf (pol). b Average restoration count count 4 Simultaneous vehicle control value
1 Canada, ULC, Charles River Laboratories in Montreal, 2 Boone, North Carolina, USA Department of Genotoxicity
It is worth noting that microbial communities in highly contaminated places are different from microbial communities in other soils. This effect of experimentally soil contaminated with metals (12, 13) and soil exposed to soil for over 20 years has been previously reported (23). The presence of Pb resistant microorganisms in the soil was also evaluated by isolation culture; however, our results could not be representative of the entire microbial community as only a small percentage of bacteria (8) could be cultivated. Surprisingly, the proportion of Pb resistant culturable population in Pb contaminated soil is not much higher than in A soil. Other studies focused on the culturable part of the microbial community suggest that 10% to 100% of long-contaminated habitat bacteria are metal resistant (19, 25)