Mapping the Genes of Sordaria Fimicola

The main purpose of this experiment was to investigate the results of wild type mutant crosses that influence asc spore arrangement in fungus Sordaria fimicola. These results are adjusted to help calculate the spectral distance between Sordaria centromere and spore color genes. My hypothesis is that the data is underestimated and the result is not suitable for chi square table because so many groups are observed. After 1 week, samples from culture dishes containing 2 mutant stock cultures were observed.

Sordariaceae is a fungal family of fungi of the ascomycetes Sordaria fimicola and is mainly used for gene localization and hybridization in the meiotic cycle (Ellis & Ellis, 1998). Most life cycles of fungi are consumed in a haploid state (El-Ani, 1967). Cells are mainly influenced by filiform haploid cells and hypha, and hyphae accumulate and mycelia form. For two combined haploid cells from two different mycelia, the nucleus contains a diploid fertilized egg. At this point meiosis occurs, four haploid cells arise, and the organism returns to its unique haploid stage. Because Sordaria fimicola forms ascospores, the resistant cell wall produces 8 haploid cells that thicken to form such resistant cell walls, so the organism undergoes mitosis (Helms). The purpose of this study was to observe the transmission of genetic material between black line and Sordaria fimicola tan line.

Many studies have examined the common fungus Sordaria fimicola as a major reliable model organism, showing its robust structure and inheritance by lifecycle. To map the distance between the tan gene (t - g +) and the centromere, it is necessary to carefully prepare a fusion sample of Sordaria already containing hybrid and non - hybrid configurations in ascus. By measuring the amount of mixed MI (non-crossing) asci and MII (crossing) asci and calculating the crossover frequency, the percentage of Asci can also be calculated from the crossover rate throughout the experiment. Through understanding of crossover frequency, biological ideologies such as adaptation, mutation and recombination are fully expressed in experiments. The null hypothesis indicates that there is no significant difference between the expected 26 map units and the observed map distance and the collected class data (Helms, Kosinski, Cummings, 350).