LC-MS and LC-MS/MS: Principles and Applications

Introduction Mass spectrometry (MS) is a method of analyzing samples and measuring their mass-to-charge ratios. It can be used to determine the quality of the sample as well as the composition of the sample. Liquid chromatography (LC), more specifically high performance liquid chromatography (HPLC) is a technique for separating a mixture of complex compounds into its constituent molecules by chromatography, Components can be identified, quantified and purified. .

Two dimensional liquid chromatography (LC) combined with mass spectrometry (LC - MS) is a major technique for high - throughput proteomics. The proteins were separated in parallel from different samples using LC and then the specifically expressed proteins identified by tandem mass spectrometry were selected and stained. MS 2 Spectrum Recognition: Supports third-party peptide search engines, custom and scalable basic search engines, indexes peptides in custom protein databases using SeqAn, combines posteriori error probability and FDR / q values ​​variously Statistically verified Peptide search results by ConsensusID search engine

Microchip chromatography is a revolutionary innovation in LC-MS technology, which integrates LC-MS's liquid handling process into a planar substrate, now known as microchip. The microchip chromatography system provides the same function as a typical LC system, but improves the limitations of common LC systems. Pharmafluidics is a world leader in microchip chromatography and offers a new approach that greatly enhances the limits of LC - MS in complex biological sample analysis. They developed a microchip chromatography device combining an ultra-high resolution uPAC HPLC column with a high precision mass spectrometer. Due to the high resolution and high sensitivity of this innovation, this device has identified more than 3,000 proteins from 50 HeLa cells.

In the past few years, bioanalytics have depended greatly on LC-MS based technology. Indeed, LC-MS technology has proven to be very effective, and by 2015 the global market value of liquid chromatography has exceeded $ 1 billion per year. Although the LC - MS method appears to be the ideal choice for biological compound analysis, LC - MS technology still faces challenges when trying to detect macromolecular drugs. These challenges include low selectivity of background interference, sub-standard data quality, lack of reproducible results.