Lab Report Using a Chemical Titration to Measure Rate of Conversion of Hydrogen Peroxide to Water and Oxygen

This laboratory reports on the use of chemical titration to measure the conversion rate of hydrogen peroxide to water and oxygen. Enzyme catalase Purpose: Before proceeding with this exercise, you should understand: Ø General functions and activities of the enzyme Ø Relationship between structure and function of the enzyme Ø How the concept of free energy relates to enzyme activity Ø Changes in pH, enzyme concentration and substrate concentration affect initial reaction

Laboratory reports using enzyme catalyzed measurements of hydrogen peroxide conversion rate essentially use chemical titration and then calculate the conversion of hydrogen peroxide (H 2 O 2) to water and oxygen using enzyme catalase It is a main purpose to do. Another purpose of the laboratory is to measure the effect of changes in temperature, pH, enzyme concentration and substrate concentration on enzyme rate. - Factors influencing the plan of enzyme activity -------- Objective --- Investigate factors that affect catalase activity while keeping all variables constant. Possible Independent Variables ------------------------------ Below are some possible independent variables that can be changed in experiments : Independent variable Easy to measure continuous / discontinuous

This laboratory reports on the use of chemical titration to measure the conversion rate of hydrogen peroxide to water and oxygen. Enzyme catalase Purpose: Before proceeding with this exercise, you should understand: Ø General functions and activities of the enzyme Ø Relationship between structure and function of the enzyme Ø How the concept of free energy relates to enzyme activity Ø Changes in pH, enzyme concentration and substrate concentration affect initial reaction

The pH of the hydrogen peroxide and the chemicals used to dilute the hydrogen peroxide (water) will affect the reaction rate. This is because the enzyme (catalase) is affected by pH as all enzymes do. The enzyme has the highest efficiency or "optimum pH", catalase also works before and after it, and it undergoes denaturation under extreme acidity or alkalinity. This is controlled using the same amount of yeast (2 cm) throughout the experiment. I also confirm that the concentration of the solution is the same by using 5% yeast solution (prepared in the laboratory). This will prevent this variable from affecting the reliability and accuracy of the experiment.