Lab Report: Identifying Biomolecules - Catherine Ruedi...

Catherine Ruedi Date: 22 February 2016 Biology 1406 Lab Report 1 Professor Kintsley identifies biomolecules In this lab, we test known and unknown substances and identify biomolecules in each substance. The three biomolecules tested were carbohydrates, proteins and lipids. This is done by using separate reagents for each individual test. This is the biological molecule that the solution has. Reagents are substances or mixtures used for chemical analysis or other reactions. Five types of reagents are used. Sudan IV, Benedict, Iodine, biuret, paper spot test. Carbohydrates are organic compounds consisting of carbon, hydrogen and oxygen. In the experiment, the reagent used is Benedict. The other biomolecule being tested is a lipid. Lipid is glycerol and three fatty acids, two reagents can be used to test lipids. The Sudan IV and paper field test is a reagent. The last biomolecule tested is an amino acid protein. The reagent for protein is biuret. In experiments we test four known solutions and two unknown solutions. There are four known substances and two unknown substances. Unknown ones are tested with each reagent to see carbohydrates in each unknown. Hypothesis: Since unknown substances appear to be separate lipids to separate, it can be deduced that lipids are contained in substances. In addition, it can also be predicted that these two unknown components contain clumps as they contain starch. Material: ● Test tube rack ● 16 test tube ● test tube rack (fixtures) ● crayon ● 450 ml beaker ● 7 disposable pipette ● boiling chip ● hot plate ● reagent bottle o iodine reagent b Benedict reagent o Sudan IV reagent o diurea Reagent o Brown paper (field test) ● Boiling chips ● Four known solutions o Starch o Sugar o Lipid o Protein

Reagents are chemicals used in our laboratory to identify different types of biomolecules. There are four types of biomolecules: carbohydrates, lipids, proteins, and nucleic acids. In this experiment we run two different carbohydrate tests, a lipid test and a protein test. Five unknown samples, each containing biomolecules, are obtained. You will react each chemical substance with each unknown sample to identify the biomolecule that exists and the given cell function of each sample

Biology 1406 Lab Report 1: pH, biomolecules known / unknown Test name: Ashley Gutierrez Lab Coach: Krista Johnson Objective: The purpose of this exercise is to test the existence of these biomolecules in known and unknown substances. When it is a biomolecule, we must find out an unknown substance. When an unknown substance is tested to find if the weather is good or bad then the presentation of that compound will be negative. Materials: • 24 tubes • Hot plate • Brown paper bag • Pipette • Chub black • Biuret • Iodine I 2 KI • Oil • Benedict • Sudan IV • Unknown liquid • Beaker • Boiling water • Distilled water Method / Procedure: A. Starch - iodide test 1) Place all the dropper of the liquid to be tested in a clean and dry test tube. 2) Add 3 drops of iodine (I 2 KI) solution to the liquid in the test tube. 3) Lightly shake the tube side.

Lecture: 3 hours Lab: 3 hours Prerequisite: Chemistry 51 or Chemistry 65 or Chemistry 101 In this course you will learn the structure, properties, and nomenclature of organic compounds and biomolecules. A simple chemical reaction was introduced. Students use the physical and chemical properties of the compound to characterize it in the laboratory. We strongly recommend that you take this course before taking a chemistry course. 211. This course will provide credits for chemistry students' learning outcomes. Students name small organic compounds, hydrocarbons, alcohols, ethers, thiols, aldehydes, ketones, carboxylic acids, esters, amines, amides. Students explain the structure of small organic compounds. 3. Students classify constitution and cubist. 4. Students will explain the structure of carbohydrates, lipids, proteins, nucleic acids and identify physical and chemical properties. Five