Isolation and Characterization of SDS-degrading Pseudomonas aeruginosa sp

Separation and identification of Pseudomonas aeruginosa degraded by SDS Malaysia Malaysia, Putra, Malaysia University chemistry and biomolecular science Department Department of Chemistry and Biomolecular Science Due to the development of industrial economy and population density, surfactants invade the aquatic environment most widely spread It becomes one of heterogeneous organisms, causing serious environmental problems.

Pseudomonas aeruginosa is a free-living bacterium commonly found in soil, water, bacterial flora in the skin and all artificial environments. Living organisms often occur on the surface of plants and animals. Pseudomonas aeruginosa is an accidental pathogen of plants. It is also an increasingly emerging clinically relevant pathogen. Organisms do not grow in the normal atmosphere and also grow in the hypoxic region to form colonies in both the natural environment and the artificial environment. Pseudomonas aeruginosa is a human opportunistic pathogen, meaning that it uses several breakthroughs in host defense to induce infection. Bacteria do not infect uninfected tissues, but if they contain tissue protection they infect bacteria. Urinary tract infections, respiratory infections, soft tissue infectious dermatitis, gastrointestinal tract infections, etc.

Pseudomonas. Pseudomonas aeruginosa is an opportunistic pathogen that may cause a fatal infection in a damaged host. This toxicity is related to the secretion of several extracellular proteins. Pseudomonas aeruginosa secretes two proteases, alkaline protease and elastase. The alkaline protease gene (apr) from P. aeruginosa IFO 3455 and PAO 1 was cloned into E. coli. A DNA fragment encoding alkaline protease (8.8 kbp) derived from PAO 1 strain was expressed in E. coli under the control of the tac promoter. The active enzyme was found to be synthesized without cell lysis and secreted into the medium.