Interferon Regulatory Factors

As mentioned in Chapter 5, stimulation of the JAK-STAT interferon alpha-dependent pathway leads to the production of two antiviral factors. The first category is a group of antiviral genes that are directed against viral infections. For example, the Mx1 gene has an antiviral effect against influenza virus, in particular avian strain (Dittmann et al., 2008). The second class of antiviral factors are transcription factors such as IRF7 which positively stimulate the IFN-α promoter and result in the production of IFN-α.

Ovarian cancer is a serious disease with a high mortality rate because there is no effective diagnosis at an early stage. IRF1, a member of the interferon-regulated transcription factor family, has been reported in several human cancer samples to prevent oncogene-mediated malignant transformation and deletion or point mutation of the human IRF gene. In this study, IRF1 silencing was used to study the mechanism of IRF1 in ovarian cancer to identify DEGs and their functional classifications that have been altered in SK-OV-3 samples. In IRF1 knockdown, 427 was observed, including 242 up-regulated genes and 185 down-regulated genes, of which TNF, CDH1, MMP2 and DKK1 were identified as central genes and in response to drugs, cells, epithelial morphogenesis pathway Plays a major role in - cell adhesion, Wnt signaling pathway and intercellular junction construction

All IFN-stimulated genes are characterized by the presence upstream of interferon-stimulated response element (ISRE). Signal transduction ultimately leads to the binding of a specific regulator to the ISRE, which stimulates the transcription of the IFN-sensitive gene via RNA polymerase II. The induced gene product then mediates anti-IFN, immune modulation, and other effects characteristically induced by IFN