Indicactor Lab: What is in each Test Tube?

There is evidence to prove this content of each tube. Test tube A: Test tube A contains water. It is a neutral fact and I can rely on it not to respond to red and blue litmus paper. Phenolphthalein also did not react with this substance. The pH is 7, neutral, and it is also proved that the material is water as water is neutral. The substance is transparent and tasteless, and all these reactions combine to give me the conclusion that substance A is water. Tube B: Tube B contains degraded milk.

Renin is an enzyme that cures milk. You can test the effect of Renin on milk in a simple experiment. 3 ml of milk was placed in 3 tubes, 4 ml of chymosin was placed in 3 tubes, and 1 milk and 1 chymosin tube were placed in each water bath for 10 min. You can use the same water bath as amylase experiment. Renin was added to each milk tube and the tube was returned to the water bath for 10 minutes. You will notice that reaction at room temperature will produce solidified milk, but the contents of the tube in a cold water bath will be somewhat hard and the contents of the tube in hot water will not cure.

We made a workstation consisting of six test tubes. We point out the measured values ​​of each tube at 1 cm and 2 cm from the bottom. Because each tube seems to need to correspond to the temperature difference used for observation in order to avoid confusion, it was decided to 0 ℃, 20 ℃, 40 ℃, 60 ℃, 80 ℃, and 100 ℃. I need to observe 0 ° C to 40 ° C, my laboratory will work with the remaining tubes.

Tube measurement - For each part of the laboratory, measure enzyme and substrate solution and pour into tube. Even if the volume of the solution is readily determined using a measuring cylinder, it is not possible to accurately measure the length (distance) of the solution. If you use a ruler, the solution measurement will be incorrect, causing errors in every part of the lab. Amount of inhibitor in solution - Part 5: For inhibitor addition, copper (II) sulfate was added to the test tube as an inhibitor, but due to the amount of inhibitor released into the inhibitor This part contradicted. Each time the solution. The dropper requires a lot of inhibitor at a time, and then requires a small amount, so it is not a reliable material in this laboratory.