Identification and Characterizaation of Three GS Isoforms

In addition to the GS02 and GS03 proteins, there are also another casein kinase II phosphorylation (positions 257-260) and protein kinase C phosphorylation (positions 322-324), as well as tyrosine kinase phosphorylation sites (positions 173-35) . 180) Prediction of GS 01 and GS 03 proteins. GS 01, GS 02 / GS 0 3 are predicted to have 8 and 7 N-myristoylation sites, respectively. The NetPhos 0 assay predicted the 9/11/11 serine, 2/3/2 threonine and 5/7/7 tyrosine phosphorylation sites of the GS 01 / GS 02 / GS 03 protein, respectively.

For those trying to read literature in this field, a bit of confusion about the identification of human myosin heavy chain isoforms and fiber types may be confusing. It is important to remember that there are only three myosin heavy chain isoforms (from the slowest to the fastest) in the limb muscles of humans. In the remainder of this article, humans do not express the fastest myosin heavy chain isoform, MHCIIb9. 9 human MHCII x / d and histochemical myosin ATPase base IIB type fiber.

Identification of different myosin heavy chain isoforms also allows classification of fiber types (Figure 2). 1 Different myosin ATPase based fibers correspond to different myosin heavy chain isoforms 1, 8 This is not the case. It is strange as the myosin heavy chain contains this site as an ATPase. The fact that each muscle fiber can contain more than one myosin heavy chain isoform accounts for the presence of myosin ATPase fiber type in addition to pure Type I, Type II A and Type II B fibers. The human genome contains at least 10 myosin heavy chain genes, but only 3 in the adult limb muscle 1 Myosin heavy chain isoforms are immunohistochemistry using anti-myosin antibody or dodecane You can analyze by. Sodium sulfate-polyacrylamide gel electrophoresis (SDS-) was used for identification. PAGE) Separation

The three first myosin isotypes identified were MHC I, MHCIIa and MHCIIb, which are isoforms I, IIA and IIB, respectively identified by myosin ATPase staining. 1, 3 and 5 respectively. Includes myosin. Thus, histochemical myosin ATPase type IC, IIC and IIAC fibers co-express MHCI and MHCIIa genes to varying degrees while IIAB fibers co-express "adjacent" (ie MHCI and MHCIIa or MHCIIa and MHCIIb) 2 . Co-expression of MHCIIa and MHCIIb genes 1 Because of its quantitative nature, the use of single-fiber electrophoretic separation (SDS-PAGE) to identify myosin heavy chain isoforms may be the best way for muscle fiber typing I do not. Electrophoretic separation allows detection of the relative concentrations of different myosin heavy chain isoforms in mixed fibers