Human Gastric Adenocarcinoma Cell Line

Introduction There are various cell lines to investigate genotoxic exposure of drugs such as human gastric adenocarcinoma cell line - TMK 1, human colon cancer - COL 2, especially human B lymphoblastoid cell line - TK 6. TK6 is heterozygous for the thymidine kinase locus from patients at risk of chronic myelogenous leukemia (CML) T cell lineage, including the Philadelphia chromosome t (9; 22) (q34; q11), chromosome 6 Cell line. Del (1) (Q21; Q32); dic (7)), and chromosome 7, such as abnormality (a) deletion of long arm) (: P13➝cen➝q32: 11.2➝centerpter) (Watanabe et al., 1995; Tomita et al., 199

Gastric adenocarcinoma is a malignant epithelial tumor derived from the glandular epithelium of the gastric mucosa. The majority of stomach cancers are adenocarcinomas (90%). Histologically, there are mainly two types of gastric adenocarcinoma (Lauren classification): intestinal or diffuse. Adenocarcinoma invades the stomach wall and tends to penetrate the muscular mucosa, submucosal tissue and then the intrinsic muscularis. Intestinal adenocarcinoma tumor cells represent irregular tubular structures with multiple layers, multiple lumens, reduced matrix ("back to back" aspect). Usually it links the intestinal dysplasia of adjacent mucous membranes. According to glandular structure, cellular polymorphism and mucus secretion, adenocarcinoma may show three differentiation degrees. The difference is a little. When mucus remains in tumor cells, it extrudes the nucleus to the periphery: "signaling cells"

The intestinal cell line HT 29-19 A is a clone derived from the human colon adenocarcinoma cell line HT 2919 and forms dense monolayer differentiated polar cells even in the presence of glucose. This cell line was cultured in Dulbecco's Modified Eagle's Medium (DMEM; Gibco Life Technologies, France) at 37 ° C. containing 10% heat-inactivated fetal bovine serum (FCS). 1 L of glutamine, 50 mg / mL of gentamicin was added in a humidified atmosphere of 5% CO 2, 95% air. Constitutively expresses the T84-DRB1 * 0401 / CIITA cell line transfected with the HLA-DRB1 * 0401 molecule and the transactivator CIITA, and the double-transfected cell line of the invariant chain and HLA-DM.8. This allowed us to analyze the expression of MHC class II molecules on IEC-derived exosomes without secondary effects by interferon g (IFN-g) at tight junction. Therefore, this cell line is used as an epithelial cell model affected by inflammatory mediators.

Originally isolated in 1977 Caco-2 cells are continuous cell lines of human epithelial colorectal adenocarcinoma cells. When grown under specific culture conditions, parenteral cell lines undergo spontaneous differentiation, resulting in monolayer cells expressing some morphological and functional characteristics of mature intestinal cells present in the small intestine It is formed. Caco - 2 cells express tight junctions, microvilli and many intestinal cell - specific enzymes and transporters (peptidases, esterases, P - glycoproteins, amino acid uptake transporters, bile acid carboxylic acids). Therefore, Caco - 2 cells are widely used as an in vitro model in the pharmaceutical industry to predict the absorption of oral medication through the human intestinal mucosa.