Exploration of Light Microscopes

The meaning of the term "exploration microscope" in optical microscopy means that the first part of the word "micro" is very small. "Scope" section means display or display. Microscopes are also tools used to magnify the image of small objects so that they can be studied. An optical microscope is a device consisting of two lenses combining an eyepiece and an objective lens, which has a larger magnification and can be realized with a single lens. The microscope also has various knobs that focus on the microscopic photos.

Most student microscopes are classified as optical microscopes. Visible light is bent through the lens system and the user can see the sample. Optical microscopy is useful for observing living organisms, but since individual cells are usually transparent, their components can not be distinguished unless they are colored with special staining. However, staining usually kills cells. The optical microscope usually used in the Academy laboratory is about 400 times. Two important parameters of the microscope are the ability to amplify and degrade. Magnification is the process of magnifying an object on the appearance. Resolution is the ability of the microscope to separate two adjacent structures. The higher the resolution, the better the sharpness and detail of the image. When studying small objects using oil immersion lenses, the magnification usually increases 1000 times.

The maximum magnification of the modern optical microscope is about 1000 times. The resolution of the microscope is limited not only by the number and quality of the lens but also by the wavelength of the light used for the lighting. The white light has a wavelength of 400 to 700 nanometers (nm). The average wavelength is 550 nm, which leads to the theoretical limit of the resolution (invisibility) of the optical microscope with white light of about 200 to 250 nm. The figure below shows two points at the detection limit, but it distinguishes between the two points. The figure on the right shows that the two points are very close and the center points overlap.