Evaluation of In Vitro and In Vivo Osteogenic Differentiation

In this study, a nanohydroxyapatite / chitosan / poly (lactide-co-glycolide) (nHA / CS / PLGA) scaffold inoculated with human umbilical mesenchymal stem cells for bone tissue engineering was used. Repair bone defect. Tissue engineering uses basic concepts from material engineering and life science to apply them to medical needs to design alternative devices with similar morphology and function as damaged tissue to enable tissue repair . Common compounds for bone tissue engineering include PLGA due to their biocompatibility, non-toxicity and relative anti-inflammatory properties.

DPSC is a type of mesenchymal stem cell in the pulp. DPSC has the potential for osteogenic and chondrogenic in vitro and can differentiate into dentin in vivo and can also differentiate into dentin-tooth like complexes. Recently immature dental pulp stem cells, a pluripotent subpopulation of DPSCs prepared using pulpal organ culture, have been identified. All pulp tissues were enzymatically digested in 3% type 3 collagenase solution for 1 hour at 37 ° C. For further culture and growth by filtration and inoculation, cells 3-20 μm in diameter were obtained. Based on this method, small populations of cells containing a high proportion of stem cells can be isolated.

After studying various methods for analyzing RNA protein directly in vivo and in vitro, biologists have developed an in vitro method called RNA Bind-n-Seq (RBNS) for 4 years I used it. It was developed by Nicole Lambert who is a postdoctoral researcher and collaborator of former Burge Lab. Lambert has tested only a small fraction of the protein so far, but it is a quantitative method to reveal low affinity and high affinity RNA-protein interactions, and one procedure step and almost all Possible RNA motifs due to the need for screening. This new study improves detection throughput and systematically investigates the binding specificity of more than 70 human RBPs at high resolution.