Enzyme Activity Essay

Sucrase has maximum activity at pH 6. Sucrase shows maximum activity at 40 ° C (104 ° F). Sucrose activity increases with increasing sucrose concentration until a plateau is reached

Effect of pH on enzyme activity Independent variables (glucose and fructose) caused by dependent variable yield. pH control variable temperature; quantity of substrate present (sucrose); sucrase + sucrose incubation time

Explain the optimum pH and sucrase activity change of sucrase activity at more acidic and alkaline pH values. The optimum pH for sucrase activity is 6, which decreases with increasing acid number and base number.

10 ° C (50 ° F) 0.006 0.006 0.007 0.006 20 ° C (68 ° F) 0.251 0.285 0.273 0.27 30 ° C (86 ° F) 0.767 0.789 0.799 0.785 Optical density 40 ° C 50 ° C (104 ° F) 122 ° F) 0.969 0.93 0.947 0.922 0.945 0.947 0.954 0.933 60 ° C (140 ° F) 0.843 0.827 0.829 0.833 70 ° C (158 ° F) 0.641 0.623 0.632 0.632

2. Please explain the optimum temperature of sucrase activity and the change in sucrase activity at low temperature and high temperature. The optimal temperature for sucrase activity is 40 ° C. Sucrase activity may decrease and degrade at higher or lower temperatures

1 2 3 Average 35 g / L 0.988 001 0.987 0.99 2 30 g / L 0.983 0.977 012 0.99 1 25 g / L 0.946 0.94 0.943 0.943 Optical density 20 g / L 15 g / L 0.93 0.861 0.922 0.86 0.908 0.848 0.92 0.856 10 g / L 0.779 0.759 0.774 0.771 5 g / L 0.513 0.523 0.506 0.514 0 g / L 0.003 0.002 0.002 0.002

Temperature affects enzyme activity up to the peak, which corresponds to optimal enzyme activity. When this maximum value is exceeded, the enzyme activity rapidly decreases. The optimum temperature of most mammalian enzymes is about 37 ° C. The inactivating effect at temperatures above 40 ° C is due to protein denaturation. The enzyme is regulated to suit the needs of different cells. If Km in the cell is lower than Km, please correct the activity change. An allosteric enzyme is an enzyme that is regulated by a substance that binds to a site different from the active center. The initial rate and allosteric enzyme curves are sigmoid colon, not hyperbolic. Enzyme activity also changes by covalent modification (eg phosphorylation)

Activity peaks at a specific temperature specific to the enzyme. This is called the optimum temperature - the temperature at which the enzyme is most active -. When the optimum temperature is exceeded, the activity of the enzyme decreases. At extreme temperatures, denaturation and activity of the enzyme stops. Catalase activity was measured and plotted over a range of temperatures in the following experiments. The substrate of catalase is hydrogen peroxide, and its decomposition products are water and oxygen. Hydrogen peroxide is a by-product of reactive oxygen species and some biochemical reactions. Since hydrogen peroxide accumulates when it accumulates, catalase protects cells from oxidative damage.