Environmental contamination with Staphylococcus aureus at a large, Midwestern university campus

Buildings of medical professional association (HPA) and non-medical professional association (NHPA) were sampled

Prevalence of similar S. aureus samples found between samples collected from HPA and NHPA buildings

Staphylococcus aureus can cause mild to severe life-threatening infections. With the advent of multidrug resistance (MDR) strains epidemiological changes in S. aureus are attracting public health concerns. The environmental surface plays an important role in expanding S. aureus. The purpose of this study was to investigate the environmental pollution and molecular properties of Staphylococcus aureus in large-scale university healthcare professional (HPA) and non-medical (NHPA) buildings.

A total of 152 environmental surface samples were collected from two HPAs and two NHPA campus buildings. Bacterial culture and diagnosis were performed using standard microbiological methods. Polymerase chain reaction was performed to detect mecA and PVL genes. All strains are spa type. A subset of isolates was characterized by multilocus locus typing (MLST). Antibiotic susceptibility test of all S. aureus isolates

The overall contamination of S. aureus and methicillin-resistant Staphylococcus aureus (MRSA) were 22.4% (34/152) and 9% (9/152), respectively. Similar pollution is observed in buildings of HPA and NHPA. A total of 17 different hot spring types were detected in 34 S. aureus isolates. Most MRSA isolates belong to the clone complex (CC). One isolate was PVL positive. Eleven different sequence types (ST) were detected from 17 isolates tested. ST8 is the most common. 12 isolates (35.3%) are MDR

About 27% (9/34) of isolates are MRSA. The highest contamination of Staphylococcus aureus is found in the contact area of ​​door handles and other masters and the human interaction in crowded environments such as academic institutions plays an important role in the diffusion of S. aureus / MRSA by inanimate objects It is suggesting that it will fulfill. Role In addition, MDR is more than one-third of isolates. These data reinforce the need to implement effective preventive strategies outside the medical environment to reduce the incidence of drug resistant S. aureus infection

The tourniquet is a potential cause of methicillin-resistant Staphylococcus aureus (MRSA), with up to 25% of the tourniquets being contaminated due to lack of hand hygiene or repeated use of contaminated tourniquets (35). In addition, reusable finger puncturing devices and blood contamination related care point testing devices (such as blood glucose meters) are associated with the occurrence of hepatitis B (4, 5, 36).

Staphylococcus aureus can cause mild to severe life-threatening infections. With the advent of multidrug resistance (MDR) strains epidemiological changes in S. aureus are attracting public health concerns. The environmental surface plays an important role in expanding S. aureus. The purpose of this study was to investigate the environmental pollution and molecular properties of Staphylococcus aureus in large-scale university healthcare professional (HPA) and non-medical (NHPA) buildings.

Staphylococcus aureus Staphylococcus aureus is generally found in the skin and nose of healthy people (about one-third of a person). Most people who are infecting the skin and nose with Staphylococcus are not adversely affected and are described as "colonization". Methicillin-resistant Staphylococcus aureus (MRSA) is a special kind of staphylococci that has become unresponsive to many commonly used antibiotics such as penicillin. These bacteria invade the body through the skin due to incision, pain or surgical incision, sometimes infection (such as impetigo, vap, abscess or infected wound). This situation is most likely to happen to people who are already sick but may also occur in healthy people in the area.