Difference Between Gram Positive and Gram Negative Bacteria

In 1884 Danish doctor Christian Gram developed a dyeing technique to distinguish between the two bacteria. Two kinds of bacteria based on Gram stain are Gram positive bacteria and Gram negative bacteria. Bacteria were first stained with Crystal Violet or Gentian Violet. All bacterial cells are stained with blue or violet in crystal violet solution. Bacterial cells were then treated with iodine solution (Lugol Iodine) solution and washed with ethanol (destaining solution). Those bacteria that retain the blue or purple color of Crystal Violet are called Gram positive bacteria and those bacteria that lose crystal violet color after washing with destaining solution are called Gram negative bacteria. Thereafter, gram negative bacteria were stained with crocin or magenta and observed under a microscope. Gram negative bacteria stained with saffron or magenta appear red or pink. Gram stain distinguishes bacteria by detecting the properties of peptidoglycan through the chemical and physical properties of the cell wall. The Gram staining method can be used to distinguish most bacterial species into two broad categories. Although it is impossible to distinguish all bacterial species based on Gram staining, this method has wide application value in clinical diagnosis and biological research.

Ø Two groups of bacteria are genetically modified through transformation, transduction, and binding

Ø Gram-positive and Gram-negative bacteria are suppressed by antibiotics (sensitivity varies)

Medical experts need to understand the important differences between gram positive and gram negative bacteria. Gram-positive bacteria are bacteria that are classified by the color that changes with the dyeing method. Hans Christian Gram developed a dyeing method in 1884. The staining method uses Crystal Violet dye which is retained on the thick peptidoglycan cell wall found in gram positive bacteria. When looking under a microscope, gram positive bacteria turn blue. Gram negative organisms typically have an outer membrane but they have a thinner peptidoglycan layer that does not retain the blue dye used in the initial dyeing process. Other information used to distinguish bacteria is shape. Gram-positive bacteria including cocci, bacilli or branching filaments

Gram positive bacteria are classified by their rotation color after chemicals called Gram stain. When the stain was applied to Gram positive bacteria, gram positive bacteria stained blue. Other bacteria stain red. They are called Gram negative. Gram-positive bacteria and gram-negative bacteria have different cell walls, so different staining. They also cause different kinds of infections, and different kinds of antibiotics are effective for them. Gram positive bacteria are increasingly tolerant to antibiotics. For example, methicillin-resistant Staphylococcus aureus (MRSA) bacteria are resistant to most penicillin-related antibiotics. Methicillin is penicillin. MRSA strains usually contain infections obtained in medical institutions and can cause infections (regional infections) obtained outside the medical institution.

The main difference between gram positive and gram negative bacteria is the difference in cell wall composition. Prokaryotes known as eubacteria have three basic forms: bacilli, cocci and spirals. Bacterial cell walls make the most important contribution to cell shape. The shape of the cell wall, presence of flagellum, if there is a location of flagella Eubacteria can be classified by Gram stain. First, gram positive bacteria are intrinsically dark blue, dark purple in nature during excessive stress. Their basic features are based on their cytoplasmic lipid membranes, cell walls, and finally the presence of bacteria in the cysts. Gram positive bacteria consist mainly of capsular polysaccharide, thick peptidoglycan layer and flagella, which are present in specific species. It can be aerobic or anaerobic in some cases