Cytotoxic and antiproliferative constituents from Dictyota ciliolata, Padina sanctae-crucis and Turbinaria tricostata

Background: Dictyota ciliolata Sonderex Kützing (Dictyotaceae), Padina sanctae-crucis Børgesen (Dictyotaceae) and Turbinaria tricostata E. S. hexane extract. Barton (Sargassaceae) was found to exhibit cytotoxic and antiproliferative activity in vitro. Bioactive compounds involved in these activities have not been studied in detail for these species, and phytochemical studies are very limited.

Objective: To isolate, evaluate and elucidate D. ciliolata, P. Physiologically active ingredients of Sanctae-crucis and T. tricostata

Materials and Methods: Bioassay-directed cytotoxic fractions of the Hep-2 cell line using hexane extracts from these brown algae were analyzed using various chromatographic techniques. The cytotoxic and antiproliferative activities of all the isolated compounds were also evaluated in panel of cell lines (KB, Hep-2, MCF-7 and SiHa). In addition, their selectivity index, the cytotoxicity ratio to normal cells and cancer cells, was evaluated using the HEK-293 cell line.

RESULTS: Four compounds were isolated from the study species: two sterols, fucosterol (1) and 24ξ-hydroperoxy-24-vinyl cholesterol (2); two diterpenoids, pakinjectiol A (3) and dictyol B acetate (4). The main bioactive component of the hexane extract of T. tricostata and P. sanctae-crucis are compounds 1 and 2 on the cell lines tested (CC50 varies from about 1 to 25.6 μg / mL). Compounds 1, 3 and 4 showed cytotoxic activity against cancer cell lines (CC50 varied between 14.8 and 41.2 μg / mL) and D. It was the major bioactive component of hexane extract of cillolata. Compounds 1 and 4 were added to MCF-7 (IC50 of compound 1 = 43.3 μg / mL, IC 50 of compound 2 = 38.3 μg / mL) and SiHa (IC50 of compound 1 = 43.3 μg / mL, compound 2) Showed anti-proliferative activity. IC 50 = 38.3 μg / mL) cell line

Conclusion: The present study is based on D. ciliolata, P. For the first time. The physiologically active components of Sanctae-crucis and T. tricostata were examined. Although compounds 1-3 were previously described, the pharmacological activity of Compound 4 is presented here for the first time.

Several diterpene skeletons from Dictyota species are characteristic constituents of this genus and have chemical taxonomic significance. For example, most of the prenylenylated guaian and dorabellantiterpenes are derived from D.I. Is isolated from dichotoma, drastone diterpenoids are found mainly in three species. Divaricato and D. linearis. Xenicane diterpenes are chemical features of the classification of Dictyota and are found mainly in only a few Dictyota species found in D. plectens.

Background: Dictyota ciliolata Sonderex Kützing (Dictyotaceae), Padina sanctae-crucis Børgesen (Dictyotaceae) and Turbinaria tricostata E. S. hexane extract. Barton (Sargassaceae) was found to exhibit cytotoxic and antiproliferative activity in vitro. Bioactive compounds involved in these activities have not been studied in detail for these species, and phytochemical studies are very limited. Materials and Methods: Bioassay-directed cytotoxic fractions of the Hep-2 cell line using hexane extracts from these brown algae were analyzed using various chromatographic techniques. The cytotoxic and antiproliferative activities of all the isolated compounds were also evaluated in panel of cell lines (KB, Hep-2, MCF-7 and SiHa). In addition, their selectivity index, the cytotoxicity ratio to normal cells and cancer cells, was evaluated using the HEK-293 cell line.

Cytotoxic and antiproliferative components from Dictyota ciliolata, Padina sanctae-crucis and Turbinaria tricostata

Marine brown algae of the genus Dictyota belong to the family Dictyotaceae and are mainly distributed in subtropical and tropical oceans. Structurally diverse secondary metabolites from members of this genus are inherently defensive and found to greatly facilitate their successful survival and reproduction in complex and diverse marine environments. Hundreds of bioactive natural products, including terpenes, phenols, sterols, fatty acids and polysaccharides, were isolated from marine brown algae of the genus Dictyota. Dioxin is a large class of diverse natural products found in marine organisms including Dictyota species. Due to its outstanding pharmacological activity, several diterpenes are promising candidates. Several diterpenoids from Dictyota species are considered to be characteristic components of genus and give taxonomic significance to them.