Comparing Free to Immobilised Amylase Enzyme in Its Catalysis Rate

Comparison of free immobilized amylase in the contact rate method: First, immobilized enzyme needs to be prepared. The method used to prepare these immobilized enzymes is microencapsulation. This means that the enzyme used in this experiment is amylase encapsulated in sodium alginate. Since this enzyme is thought to act very quickly, it is necessary to slightly dilute the enzyme to get good results. 0 cm 3 of amylase was added to 20 cm 3 of sodium alginate and then mixed.

Factors affecting the rate of enzyme catalysis All organisms have enzymes. They consist of amino acid polymers and are produced in living cells. Each cell contains hundreds of enzymes that catalyze multiple chemical reactions. Enzymes are known as biocatalysts because they significantly increase the rate at which reactions occur in vivo without "exhausting" or otherwise affecting the reaction. - Starch hydrolyzing enzyme is the second most important commercially available enzyme, accounting for 30% of global enzyme consumption and accounting for 25% of the world's enzyme market share. Among them, α-amylase is the most important starch hydrolyzing enzyme and is used in a wide range of applications in starch processing, breadmaking, automatic dishwashing detergents, textile desizing, medicine, pulp and paper, brewing industry .

The purpose of this experiment was to study the enzymatic kinetics of the starch hydrolysis reaction with the enzyme, alpha-amylase and to determine the kinetic effect of the alpha-amylase concentration change on the reaction rate over time there were. As shown in the table, the experiment was carried out in three main phases. For alpha-amylase concentration, experimental data on the time course of the reaction between the alpha-amylase and the starch-iodine complex were obtained at 0.02% w / v, 0.01% w / v and 0.005 It was. Absorbance of% w / v starch-iodine complex was read spectrophotometrically every 5 minutes every 5 minutes. A series of calculations were performed to infer the amount of product produced and a progress curve was generated for each alpha-amylase concentration during the reaction, in the figure, the initial reaction rate = 0 to time = 2 is the tangential line from time Can be plotted. Then calculate the gradient