Cloning and Sequencing a Portion of Glyceraldehyde-3-Phosphate Dehydrogenase Gene from the Leaves of Soleirolia Soleirolii

Cloning and sequencing of the glyceraldehyde-3-phosphate dehydrogenase gene from leaves of Soleirolia Soleirolii December 13, 2013

Introduction Without energy, plants no longer exist. Energy is an important part of the photosynthesis process. In this photosynthetic pathway, glycolysis contributes to the production of ATP and NADH. ATP described by glycolysis is energy that can be reused for another photosynthesis. The glyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene is important in the glycolysis process.

The glyceraldehyde-3-phosphate hydrogenease gene (GAPDH) is an important enzyme housekeeping gene that catalyzes an important step in glycolysis and is found in all phylogenetic development. Genes can be extracted and isolated from plant gDNA using PCR. After cloning, the GAPDH gene is sequenced and finally analyzed by bioinformatics for further study. The grass studied through the experiment is as follows. Known as kangaroo glass Themeda Triandra is native to Australia and grown in all states and regions (Unkown, nd. Native Seeds). This grass is a perennial cluster growing to a height of 5 meters and a width of 0.5 meters. This special grass is gray / green leaves very rough, with seeds in their heads to make a very distinctive red / brown spike (Jennifer Liles, 2004).

Glucose-6-phosphate dehydrogenase (G6PD) is an enzyme of the pentose phosphate pathway (see image, also known as HMP shunt pathway). G6PD is the rate-limiting enzyme of this metabolic pathway that converts glucose-6-phosphate to 6-phosphoglucono-δ-lactone and reduces cells by maintaining reduced coenzyme nicotinamide adenine dinucleotide phosphate. Energy (NADPH) NADPH then maintains a supply of reduced glutathione within the cell for removal of free radicals that cause oxidative damage

Glucose-6-phosphate dehydrogenase is the first step in the pentose phosphate pathway, a series of chemical reactions that convert glucose, the sugar found in most carbohydrates, to another sugar, ribose-5. - phosphoric acid. Ribose-5-phosphate is an important building block of DNA and its chemical epitope RNA. This chemical reaction produces a molecule called NADPH that acts to protect cells from potentially harmful molecules called reactive oxygen species. These molecules are byproducts of normal cell function. Compounds produced by reactions involving NADPH prevent the formation of reactive oxygen species at toxic levels within the cell. Glucose-6-phosphate dehydrogenase produces NADPH, which is essential for erythrocytes, but because red cells lack other enzymes that produce NADPH, it is particularly vulnerable to destruction by reactive oxygen species.