Chromatography Of Candy Dyes

Introduction: Edible dyes are naturally derived originally used for low quality and food deterioration. For a long time scientists began exploring ways to artificially dye foods in the 1800s and when people started eating these colored foods, this unfortunately resulted in a lot of injuries and deaths It was. Today, the color used for food must be regulated by the government before it is used. These synthetic food dyes are added to many kinds of foods to increase their visual appeal; most people know their use in candies and sodas, but like cheese or butter You may not know their usage in food. And many other ready-made foods.

Candy chromatography is an analytical method for analyzing and comparing dyes used for coloring confectionery and food. It is also a proven way to analyze candy and its complex structure without tasting it. These complex mixtures can be separated into pure materials. In order to find the ingredients of your favorite candy, chromatography helps to define each ingredient in the candy sample. The word chromatogram comes from two Greek words, "chroma" stands for color and "graphics" for writing. Paper chromatography is the best separation method in candy chromatography

The purpose of this experiment was to use paper chromatography to find out which dyes were used in the coating of colored candies. The main finding is that the dye is the same color as candy and supports this assumption. However, some sweets consist of a mixture of dyes, and when the color moves over the paper, the color of the mixture separates. This only partially supports this assumption. However, these are not the only reasons to separate the mixture. First, solubility: If the components of the mixture are soluble in the solvent used, the mixture is carried on the tape as the solvent flows.

Column chromatography is used to separate mixtures of various ingredients to prepare separate solutions. From the results obtained, it can be seen that the methylene blue dye component was separated from the dye mixture by the 95% ethanol solvent as it elutes the blue solution, but the fluorescein dye was not completely replaced by the solvent. Yellow Mark This is because methylene blue is a slightly basic compound as indicated by its structure (see Question 2), which is attracted to slightly acidic ethanol. (CITE) In addition, the 0.1 M NaOH solvent successfully eluted the fluorescein dye as well as the methylene blue dye as it eluted on the blue dye and yellow dye streams forming a yellow-green compound. This proves that polarity is lower than NaOH because 95% ethanol can elute only one of the two dyes (using two chromatographic methods).