Chromatography Experiment

The execution of the chromatographic experiment I follow changed in many ways. These changes are necessary to improve the experiment and improve the results. Therefore, the actual implementation method is explained below. First take the chromatographic paper and draw a line 2 cm above the bottom of the page. This is the origin. A cross is drawn on the line, which becomes the starting point of the sample.

Introduction: The purpose of this experiment is to demonstrate various chromatographic techniques for analyzing the composition of complex mixtures. The laboratory uses thin layer chromatography and column chromatography to separate components of the mixture. This is a 0.2% fluorescein dye in 95% ethanol and a (1: 1) solution of 0.2% methylene blue. Both methods are based on the differential distribution of the mixing ratio between the two phases. These two phases include a stationary phase and a mobile phase.

Chromatography is a technique for separating complex mixtures between stationary and mobile phases. There are several types of (Craig) chromatography: gas chromatography, liquid chromatography, ion exchange chromatography, affinity chromatography, adsorption chromatography, partition chromatography and size exclusion chromatography. (Carrier, Bordonaro, and Yip) The idea behind this process is that the smaller the affinity of the molecule for the stationary phase is, the faster the movement is. The stationary phase depends on the type of chromatography used (Carrier, Bordonaro, Yip). In most cases, chromatography is used for purification techniques. This method is also used for substance separation, an example of which is plant pigment.

Planar chromatography is a separation technique in which the stationary phase is present as a plane or exists on a plane. The plane may be a layer of solid particles spread on a support such as paper (paper chromatography) or a glass plate impregnated with a material as a fixed bed, as such or as a fixed bed (thin layer chromatography). Different compounds in the sample mixture move different distances depending on the strength of the interaction with the stationary phase compared to the mobile phase. The specific retention factor (Rf) of each chemical is useful for identifying unknown compounds.

Another technique used in this experiment is gas chromatography (GC). GC is the separation of compounds between stationary phase and mobile phase. GC can also be referred to as gas chromatography (VPC) and gas liquid chromatography (GLC). The sample enters GC from the inlet. The two components A and B differ in that they adhere to the absorbent and are separated (VPC). Several absorbers are coated in the liquid phase. The different components of the injected mixture will take longer in the liquid phase than others. Again, there is separation. This technique is called (GLC). The sample moves from the column to the detector. Here, the presence of the sample is detected and it is substantially converted to the GC peak. Equipment usually has two detectors. When only the gas passes through the two detectors, the circuit is considered to be in equilibrium, there is no signal so there is no movement of the pen.