Biology: Determining the Lengths of Telomeres Using Flow FISH Method

Flow FISH is a method for determining the telomere length, which is currently common due to its versatility, it can handle samples containing faster and smaller amounts of cells (Lansdorp, 1996). The main limitation of the flow FISH is that it requires expensive analysis of the device and the probes used for device configuration (flow cytometry) and hybridization are also very expensive and require trained personnel It is to be. Flow - FISH involves hybridization of fluorescently labeled peptide-nucleotide probe having a sequence complementary to telomere DNA of fixed sample cells and telomere repeat DNA sequence.

Several methods including Southern blotting, flow FISH and qPCR techniques are available for measuring telomere length. The first two methods are boring. The qPCR method is fast and measures the relative telomere length (relative T / S ratio) by comparing the fluorescence signal obtained by annealing the primer to the telomere repeat sequence with the signal generated by single copy gene amplification to enable. Especially due to development factors or aging in the kidney cortex, telomere shortens with age of kidney. Melk et al. Studied the relationship between telomere length and GFR and age as these measurements decreased with age. There is a weak positive correlation between GFR and telomere length in the kidney cortex. GFR is stable from 30 to 40 years old, then linearly decreases at an average rate of about 8 ml / min / 10 years. The length of telomere gradually decreases during aging. Telomere shortening is related to organs (eg:

Unfortunately, there seems to be no simple direct relationship between telomere length and age of organisms. In general, telomere will shorten with age in most taxa. However, the telomere length of two aged individuals of the same species is highly variable, suggesting that genetic, different cell division rates, levels of telomeric specific DNA damage, or how cells respond to differences in telomere shortening And it depends on the organization. For example, your father at the age of 70 depends on the above factors, but some organizations have telomere longer than 30 years old.

Dr. Elizabeth Blackburn and Dr. Elissa Epel have identified their "telomere effect", the lifestyle and physiology of the telomere head, in part of the chromosome that determines our age. As the length of telomeres decreases, we become more susceptible to disease. These Nobel laureate doctors have proved that our habits can control the length of telomere. Knowing the choices of food you eat, your physical activity, the environment you expose yourself, and your daily lifestyle may change your health at a genetic level you may make you scared not. Although not an absolutely sure solution, making a smart choice in these areas of life will help you to lead a healthier life.