Aseptic Transfer Techniques, Culture Characteristics and Colony Counting of Bacteria

Objective: The objective of this experiment was to transfer the medium aseptically to a test tube, to move Serratia marcescens cells from broth culture, to move Sm ramp cells, and to understand the grapes and agar in the broth Met. M. Aureus, Bacillus subtilis and M. The growth pattern of smegmatis, and E. coli. E. coli colonies were counted on the plate, and therefore the number of cells present on each plate was determined. Information and method: Please refer to the handouts. Place 0 mL of liquid medium in 3 sterile tubes.

Sterile technology is the foundation in the field of microbiology and is an important testing technology. Microbiologists use aseptic techniques to perform a variety of operations such as transfer of culture fluids, inoculation of media, separation of pure culture fluids, and conducting microbiological tests. Proper sterilization technology prevents culture from contaminating foreign environmental bacteria. For example, it is found in microorganisms (including fungus) in the air, microorganisms picked up from the body of the researcher, laboratory countertops or other surfaces, microorganisms found in dust, and non-sterilized glassware and equipment Microorganisms contaminate the culture. Objects, thereby interfering with inspection results. With proper sterilization techniques, the risk of contamination can be greatly reduced and even eliminated. In addition, aseptic technology is essential to maintaining a pure culture and transferring that culture to new media. This is especially important when dealing with pathogens

Experiment: 3 hours This course is a study of techniques and procedures that are regularly used at the Institute of Microbiology. This includes laboratory safety and sterility techniques, preparation of media, training of handling and maintenance, and use and maintenance of laboratory equipment, especially microscopes. This course is designed for chemistry, process and bio manufacturing engineers. Student learning outcome: 1. Prove knowledge on laboratory safety technology and standards, and use it properly in the laboratory. Isolation and transfer of pure cultured bacteria under sterile conditions. 3. Prove knowledge about bacteria and fungi commonly found in microbial laboratories. 4. Stain bacterial smears with brightfield microscope for preparation and observation. Determine bacterial population density using standard plate count and turbidimetric techniques. 6. Prepare different types of liquid and solid media. 7. Prepare the bacterial culture and store it correctly. 8. 9