Antibody Modeling Techniques

Modeling Antibodies Antibody modeling technology has been going through a long way since its founding. The initial insight into the antibody sequence - structural relationship can be attributed to pioneering studies of Wu and Kabat which identified the six hypervariable regions on the heavy and light chains and correctly predicted the presentation of these regions (Wu and Kabat 1970). It is named "complementarity determining region" (CDR), in contrast to the surrounding framework region (FR), by a relatively conserved framework and by carrying out the selective binding of antigens.

The specificity of binding between the antibody and the antigen makes the antibody an excellent tool for detecting substances by various diagnostic techniques. Antibodies specific for the desired antigen can be conjugated to isotope (radioactive) or fluorescent labels or chromogenic enzymes to detect it. However, similarity between some antigens may lead to false positives and other errors In these tests, antibodies cross-react with antigens that are not completely matched.

Sandwich ELISA is a technique for detecting antibodies or antigens present in the patient's blood. This technique is also known as a capture method to detect the antigen level between two layers of antibodies. The antigen measured in this technique should contain at least two antigenic epitopes capable of binding to the antibody. Sandwich ELISAs have many advantages such as high specificity, flexibility and sensitivity. ] 3, 8 [The monoclonal mouse anti-rabbit IgG with the six curves obtained from the results shown in Figure 1 showed different strict dilution ratios (1: 2000, 1: 4000, 1: 8000, 1: 16000, 1: 32000) which has the same name. , 1: 64000). According to my results, 1: 2000 dilution rapidly increases and consumes more antibody, but this is not recommended. As the antibody decreases it gradually increases, the optimal dilution is 1: 4000, this dilution can detect the lowest concentration of antigen and can be used for more samples.