An Investigation into the Effect of Lipase Concentration on the Hydrolysis Of Fats

A study of the effect of lipase concentration on fat hydrolysis used a data logger to record the pH every 5 seconds and for each experiment the data logger graphed a pH change. The slope is calculated from each of these figures, which shows the rate of change in pH per second. First, select the steepest part of the chart and calculate the gradient by dividing the pH change of that part by time. However, this method is very inaccurate and the results are very different, eg in these results the average reaction rate of the 4% lipase solution (about 0.457 pH / min) is less than 3%.

The graph uses the enzyme research program to calculate the effect of temperature on reaction rate in enzyme lipase simulation. The reaction rate of the lipase at different temperatures is based on a pH value of 9, a substrate concentration of 0.01 mol / L, and a constant variable enzyme concentration of 1 × 10 -6 mol / L. As shown, the optimum temperature of the lipase is 37-38 ° C. Actual experimental results on the influence of pH on enzyme catalase kinetics were evaluated using computer software Logger Pro and each experiment was evaluated at 3 minute intervals. The reaction rate of catalase varies from pH 3 to 11 and is a constant variable based on 3 mL 3% H 2 O 2 and 3 drops enzyme concentration.

In this experiment, we evaluate the influence of environmental factors using actual catalase analysis experiment and simulation experiment of lipase. Catalase is an enzyme that converts hydrogen peroxide, a harmful byproduct of metabolism, into oxygen and water, and it exists in all living cells. The enzyme catalase binds to the substrate and the hydrogen peroxide reacts with the catalyst to produce water and oxygen. However, lipases break down into lipid or other fat absorbable forms and are enzymes present in the pancreas or small intestine. Enzyme lipase binds to its fat, fat, catalyzes the reaction and breaks it into glycerol and fatty acids. Catalase is expected to function best at a temperature of 37.5 ° C., pH of 8, and high enzyme concentration and substrate concentration.

Oils and fats may be involved in various chemical reactions. For example, since triglycerides are esters, they can be hydrolyzed in the presence of certain enzymes called acids, bases, or lipases. Hydrolysis of fats and oils in the presence of base is used to make soaps known as saponification. Today, most soaps are prepared by hydrolyzing triglycerides (usually from butter, coconut oil or both) using water at high pressure and high temperature. Next, convert the fatty acid to its sodium salt (soap molecule) using sodium carbonate or sodium hydroxide.