Amylase Investigation

Amylase survey * It is predicted that reaction rate increases as the temperature rises. I believe that the reaction rate drops dramatically once it reaches a certain temperature. This is because most chemicals react faster when the temperature is high. At higher temperatures, the molecules rotate faster, which makes them more responsive together. Typically, an increase of 100 ° C. would double the reaction rate. This applies to enzymes up to about 40 ° C.

The figure shows the relationship between the reaction rate and temperature of human amylase. Data were collected from simulation experiments using program enzyme studies. In this experiment, the reaction rate of human amylase was 0.01 mol / L based on the substrate concentration, the enzyme concentration was 0 to 10 -6 mol / liter, and the optimum temperature of human amylase was 400 ° C. Met. The figure shows the relationship between the reaction rate of human amylase and pH. Data were collected from simulation experiments using computer software Enzyme Investigations. When the temperature is 250 ° C., the substrate concentration is 0.01 mol / l and the enzyme concentration is 0 × 10 -6 mol / l, the pH varies between 1 and 14.

To investigate the effect of temperature on enzymatic activity, use enzyme amylase which is used as a biocatalyst for degrading starch which can not pass through the intestinal wall due to its molecular size and becomes smaller starch. Amylase is a sugar enzyme that converts starch into monosaccharide in the salivary gland. The three features of all enzymes are as follows. They are always proteins. The enzyme works by continuously moving and colliding with the substrate. Each enzyme has a specific substrate. When it collides with the correct one, the substrate refers to a recess (also known as a known recess) as the "active part". The reaction then occurs and the reaction product leaves the active site. The entire process is repeated