Affect of the Rate of Reaction of Amylase on Starch and How Its Affected by the Concentration of the Substrate

Reaction rate of amylase to starch and its influence by matrix concentration list Ø test tube Ø measuring cylinder Ø thermometer Ø 3 pipette Ø starch solution Ø amylase Ø iodine solution Ø water Ø aquarium Ø spot tile Ø goggles health and safety ====== ========== Overhang remove all dangerous gems such as earrings, bracelets, chains. Make sure that the clothes do not disperse your clothes such as ties and scarves and make sure that all the long hair are tied together.

Reaction molecules in the enzymatic reaction are called substrates. The purpose of this experiment was to determine the maximum rate and substrate concentration of alpha-amylase and starch reactions. After combining the substrate (starch) and the enzyme (- amylase) for a short time, decompose it to make the product and the enzyme. As the enzyme structure changes during the reaction, it is useless and degrades in the normal course of cellular metabolism. The rate of enzymatic reaction required in FIGS. 3 and 4 is determined by the rate at which the enzyme-substrate complex is formed and then decomposed to form the product. The time required for the enzyme to activate the substrate and the frequency of complex (enzyme-substrate) formation. The frequency depends greatly on the concentration of the matrix. The maximum reaction rate is highest at this stage of the reaction, since most of the starch is converted in the early stages of the reaction.

The concentration of the enzyme and the substrate also influence the reaction rate, but it does not affect the structure of the enzyme. If the substrate concentration is constant, the reaction rate is also constant. However, as the substrate concentration increases, the reaction rate increases until it reaches the level of enzyme saturation, which means that the enzyme is currently acting on the substrate, which is limited by the amount of enzyme. Furthermore, as the substrate is constant and the enzyme concentration increases, the reaction rate also increases.

Substrate concentration and enzyme concentration affect the reaction rate of the enzyme. As the substrate concentration increases, it means that more substrate is added and the reaction rate increases with the use of more active sites of the enzyme (Enzyme 2007). However, when the active site of the substrate reaches another point, the enzyme saturates and limits the reaction rate. Therefore, if the substrate concentration is constant, the reaction rate is constant. However, if the substrate remains constant, the enzyme concentration increases and the reaction rate increases to some critical concentration.